54459
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54459
Sigma
HEPES
BioUltra, ≥99.5% (T)
| Synonym: | 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) |
| CAS Number: | 7365-45-9 |
| Linear Formula: | C8H18N2O4S |
| Molecular Weight: | 238.30 |
| Beilstein Registry Number: | 883043 |
| EC Number: | 230-907-9 |
| MDL number: | MFCD00006158 |
| PubChem Substance ID: | 24878775 |
Description
| Other Notes | One of the best general purpose buffers available for biological research. Has been used to advantage in tissue culture, oxidative phosphorylation, protein synthesis with cell-free bacterial systems, photophosphorylation, CO2 fixation1,2; Suitable buffer for TEM studies (Transmission Electron Microscopy) as it does not affect metal substrates3; Recommended buffer for the glutamate binding assay, prevents binding to non-receptor materials4. |
| Easily compare specifications for HEPES products with the HEPES specification table. |
Properties
| product line | BioUltra |
| assay | ≥99.5% (T) |
| total impurities | insoluble matter, passes filter test |
| ign. residue | ≤0.1% (as SO4) |
| loss | ≤0.5% loss on drying, 110 °C |
| pH | 5.0-6.5 (25 °C, 1 M in H2O) |
| useful pH range | 6.8 - 8.2 |
| pKa (25 °C) | 7.5 |
| solubility | H2O: 1 M at 20 °C, clear, colorless |
| anion traces | chloride (Cl-): ≤50 mg/kg |
| sulfate (SO42-): ≤200 mg/kg | |
| cation traces | Al: ≤5 mg/kg |
| As: ≤0.1 mg/kg | |
| Ba: ≤5 mg/kg | |
| Bi: ≤5 mg/kg | |
| Ca: ≤10 mg/kg | |
| Cd: ≤5 mg/kg | |
| Co: ≤5 mg/kg | |
| Cr: ≤5 mg/kg | |
| Cu: ≤5 mg/kg | |
| Fe: ≤5 mg/kg | |
| K: ≤50 mg/kg | |
| Li: ≤5 mg/kg | |
| Mg: ≤5 mg/kg | |
| Mn: ≤5 mg/kg | |
| Mo: ≤5 mg/kg | |
| Na: ≤50 mg/kg | |
| Ni: ≤5 mg/kg | |
| Pb: ≤5 mg/kg | |
| Sr: ≤5 mg/kg | |
| Zn: ≤5 mg/kg | |
| λ | 1 M in H2O |
| UV absorption | λ: 260 nm Amax: 0.045 |
| λ: 280 nm Amax: 0.035 |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 1 |
| RTECS | TL6809000 |
References
| Cited Reference | 1. N.E. Good et al. Biochemistry 5, 467, (1966) Abstract |
| 2. N.E. Good, S. Izawa Meth. Enzymol. 24, 62, (1972) | |
| 3. J.A. Panitz et al. J. Electron Microsc. Tech. 2, 285, (1985) | |
| 4. M. Ito et al. Science 38, 1089, (1986) | |
| Merck | Merck 13,4672 |
| Beilstein | Beil. 23,V,2,379 |
